Search results for "Functional protein"
showing 4 items of 4 documents
Engineered Functional Redundancy Relaxes Selective Constraints upon Endogenous Genes in Viral RNA Genomes
2018
Functional redundancy, understood as the functional overlap of different genes, is a double-edge sword. At the one side, it is thought to serve as a robustness mechanism that buffers the deleterious effect of mutations hitting one of the redundant copies, thus resulting in pseudogenization. At the other side, it is considered as a source of genetic and functional innovation. In any case, genetically redundant genes are expected to show an acceleration in the rate of molecular evolution. Here, we tackle the role of functional redundancy in viral RNA genomes. To this end, we have evaluated the rates of compensatory evolution for deleterious mutations affecting an essential function, the suppr…
Enforcing Conceptual Modeling to improve the understanding of human genome
2010
It is widely accepted that the use of Conceptual Modeling techniques in modern Software Engineering leads to a more accurate description of the problem domain. The application of these techniques in the context of challenging domains as the human genome is a fascinating task. The relevant biological concepts should be properly addressed through the creation of the corresponding conceptual schema. This schema will improve the description of the global process followed from a DNA sequence to a fully functional protein. Once the conceptual model is established, the corresponding database is created. The database is intended to act as a unified repository of integrated information that will all…
Multi-functional Protein Clustering in PPI Networks
2008
Protein-Protein Interaction (PPI) networks contain valuable information for the isolation of groups of proteins that participate in the same biological function. Many proteins play different roles in the cell by taking part in several processes, but isolating the different processes in which a protein is involved is often a difficult task. In this paper we present a method based on a greedy local search technique to detect functional modules in PPI graphs. The approach is conceived as a generalization of the algorithm PINCoC to generate overlapping clusters of the interaction graph in input. Due to this peculiarity, multi-facets proteins are allowed to belong to different groups correspondi…
Production of soluble eukaryotic recombinant proteins in E. coli is favoured in early log-phase cultures induced at low temperature
2013
Abstract Background Producing recombinant plant proteins expressed in Escherichia coli produce in high yields and in a soluble and functional form can be difficult. Under overexpression conditions, proteins frequently accumulate as insoluble aggregates (inclusion bodies) within the producing bacteria. We evaluated how the initial culture density, temperature and duration of the expression stage affect the production of some eukaryotic enzymes in E. coli. Findings A high yield of active soluble proteins was obtained by combining early-log phase cultures and low temperatures for protein induction. When IPTG was added at OD600 = 0.1 and cultures were maintained at 4°C for 48-72 h, the soluble …